综合功能实验结果，本工作最终发现了3个未知的位于细胞膜上的GLP-1R调控因子（SLC4A4，ATP1B3，GPC4）。最后，研究者在INS-1E β细胞中完成了时间分辨的邻近标记组学实验，在多时间点上追踪受体激活与内吞过程中的膜蛋白互作组变化，构建了内源GLP-1R的动态细胞 ...

Cargo that has been internalized by endocytosis is often recycled to the plasma membrane after being sorted in endosomes. Many proteins and lipids of the plasma membrane are internalized using ...

In addition to the classical clathrin-dependent mechanisms of endocytosis, there are several pathways that do not use a clathrin coat and are, therefore, referred to as clathrin-independent (CI ...

Conserved proteins drive clathrin-mediated endocytosis (CME), which from yeast to humans involves a burst of actin assembly. To gain mechanistic insights into this process, we performed a side-by-side ...

Clathrin-mediated endocytosis in budding yeast requires the formation of a dynamic actin network that produces the force to invaginate the plasma membrane against the intracellular turgor pressure.