Open-Source package for segmentation and analysis of gel electrophoresis images using machine learning. Both a python framework for training/running models and a QuPath GUI extension are available.
Identification of some of these discriminant proteins was performed with liquid chromatography (LC)-MS/MS of a trypsin digest of proteins purified by sodium dodecyl sulfate (SDS)-polyacrylamide ...
The imaging channels were set at 570–620 and 450–500 nm, respectively. Separate protein samples using SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis). Transfer the proteins onto a ...
ExoSap-IT kit (Thermo Fisher) was used to purify the dsDNA amplicons before moving on the next stages. Verification of the occurred amplification and of the purity of the amplified dsDNA was performed ...