Two-photon excitation microscopy - Wikipedia
Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.
双光子显微镜介绍（Introduction of two-photon Microscopy)
双光子显微镜介绍（Introduction of two-photon Microscopy) 树木里寄宿着生命，树木正在对我倾诉。. 双光子显微镜的工作原理如上图，感觉非常简单，就是用两个光子代替一个光子，产生荧光激发，听起来没有什么特别的，但是这样做却带来四点好处：. （1）样品中的 ...
Two-Photon Excitation Microscopy and Its Applications in …
Besides the discovery of fluorescent proteins, perhaps the most significant advance in bio-imaging of the last 25 years was the invention, in 1990, of two-photon excitation (2PE) microscopy . Since then, 2PE has become widely popular for in vivo imaging of neuronal structure because of its superior depth penetration and reduced photobleaching ...
More than double the fun with two-photon excitation microscopy …
2024年3月26日 — Two-photon excitation (TPE) laser scanning microscopy (LSM) has evolved from a custom tool to a broadly available imaging modality in the life sciences. Number of users and applications have...
Scanless two-photon voltage imaging | Nature Communications
2024年6月14日 — Two-photon voltage imaging has long been heralded as a transformative approach capable of answering many long-standing questions in modern neuroscience. However, exploiting its...
Two-photon calcium imaging of neuronal activity - Nature
2022年9月1日 — In vivo two-photon calcium imaging (2PCI) is a technique used for recording neuronal activity in the intact brain. It is based on the principle that, when neurons fire action potentials,...
双光子激发显微镜 - 维基百科，自由的百科全书
双光子激发显微镜（英語： Two-photon excitation microscopy ）是一种荧光成像技术，可以对活体组织进行深度约1毫米的成像。 它不同于传统的 荧光显微镜 ，其中激发波长短于发射波长，因为两个激发光子的波长长于所得发射光的波长。
Fast, cell-resolution, contiguous-wide two-photon imaging to …
2021年4月19日 — The result is a practically aberration-free, fast-scanning high optical invariant two-photon microscopy (FASHIO-2PM) that enables calcium imaging from a large network composed of ∼16,000 neurons at 7.5 Hz from a 9 mm 2 contiguous image plane, including more than 10 sensory-motor and higher-order areas of the cerebral cortex in awake mice ...
Two-Photon Imaging - SpringerLink
2022年11月30日 — This chapter will provide an overview of two-photon microscopy from elements of the theory underpinning fluorescence phenomena to functioning principles of a two-photon microscope including step-by-step practical advice on how to conduct an experiment using a two-photon microscope.
Principles of Two-Photon Excitation Microscopy and Its …
2006年6月15日 — Two-photon excitation (2PE) laser scanning microscopy allows high-resolution and high-sensitivity fluorescence microscopy in intact neural tissue, which is hostile to traditional forms of microscopy.