Phosphopeptides are modified self antigens which may induce an immune response. Protein phosphorylation is a very important and frequent post-translational modification that can impact a protein's localization, stability, and whether or not it can …

Phosphopeptide Antigens. Phosphopeptides, presented by class I or II MHC molecules, are derived from self-proteins whose phosphorylation is related to oncogenic activation. T cells reactive to some of these peptides have cancer-specific reactivity. 40,41 A first-in-humans trial testing two of them has been initiated (NCT01846143). More data are ...

2020年9月8日 · A critical review of method development in phosphopeptide enrichment. Existing methods include IMAC, MOAC and other techniques. Modifications on working condition/supporting ligand/loading capacity.

2023年6月23日 · Here we describe a mechanism by which an HLA-B*0702-specific acute myeloid leukemia phosphoneoantigen, pMLL 747–755 (EPR (pS)PSHSM), is recognized by a cognate T cell receptor named TCR27, a...

A phosphopeptide is a peptide that has undergone phosphorylation, a crucial protein modification involving the addition of phosphate groups to specific serine, threonine, or tyrosine residues, enabling the identification of phosphorylated residues in a …

Here we review the increasing knowledge gathered about the mechanisms behind the fragmentation of phosphopeptide ions and describe additionally recent advances made to improve and facilitate the identification and site localization of phosphorylated peptides.

In this article, we describe MS methods for characterizing phosphorylation sites within individual proteins as well as entire proteome samples. The utility of these methods is illustrated in examples that show the information that can be gained using these MS techniques.

To systematically investigate four TiO 2-based phosphopeptide enrichment methods, four different non-phosphopeptide excluders were used in the sample loading buffer (glutamic acid, lactic acid, glycolic acid, and DHB) to perform a comparison experiment.

2020年9月8日 · The first half of this review contains a tutorial for new potential phosphoproteomic researchers; discussing the key steps of a typical phosphoproteomic experiment used to investigate canonical phosphorylation sites (serine, threonine and tyrosine).

After binding, the enriched phosphopeptides are released from the metal ions using alkaline buffers of pH 10-11, EDTA, or phosphate-containing buffers. Here we describe a protocol for IMAC using Fe(3+) for phosphopeptide enrichment. The principles are illustrated on a semi-complex peptide mixture.