Cytomegalovirus - Wikipedia
The CMV promoter is commonly included in vectors used in genetic engineering work conducted in mammalian cells, as it is a strong promoter and drives constitutive expression of genes under its control.
CMV promoter Sequence and Map - SnapGene
SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. The maps, notes, and annotations in the zip file on this page are copyrighted material.
Engineering of the CMV promoter for controlled expression of ...
2022年4月28日 · Our study shows for the first time that novel, compact CMV-derived promoters can be engineered that exhibit up to 50% higher transcriptional efficiency (activity per unit DNA sequence) or 14% increase in total activity compared to the wild-type counterpart.
Use of the cytomegalovirus promoter for transient and stable …
ES cells can be easily manipulated at the DNA level, allowing both transient and stable expression of complementary DNA encoding transgenes of interest. The human cytomegalovirus (CMV) immediate-early enhancer and promoter is commonly used for transient expression of transgenes in ES cells.
Using a Commonly Down-Regulated Cytomegalovirus (CMV) Promoter …
Vectors are widely used to drive gene expression using a promoter. However, not all promoters are able to drive ectopic gene expression efficiently, including CMV promoter. Here, we report our data using CMV promoter for high-level gene expression in a B lymphoma cell line DG75.
Neuronal Activation Stimulates Cytomegalovirus Promoter-Driven ...
The cytomegalovirus (CMV) immediate early promoter has been extensively developed and exploited for transgene expression in vitro and in vivo, including human clinical trials. The CMV promoter has long been considered a stable, constitutive, and ...
Engineering of the CMV promoter for controlled expression of ...
Our study shows for the first time that novel, compact CMV-derived promoters can be engineered that exhibit up to 50% higher transcriptional efficiency (activity per unit DNA sequence) or 14% increase in total activity compared to the wild-type counterpart.
We demonstrate, for the first time, that the wild-type CMV promoter can be re-engineered specifically for HEK293 cells to derive highly compact and transcriptionally efficient novel promoters with increased transcriptional activity.
CMV promoter mutants with a reduced propensity to productivity loss …
2015年11月19日 · We discovered that the single mutation of C-179 to G can significantly stabilise the production of recombinant protein under control of hCMV-MIE in permanently transfected CHO cells.
High-throughput 5′ UTR engineering for enhanced protein
2021年7月6日 · Here, we develop a high-throughput strategy to design, screen, and optimize 5′ UTRs that enhance protein expression from a strong human cytomegalovirus (CMV) promoter.