Luciferase - Wikipedia
Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively. [1]
Luciferase: A Powerful Bioluminescent Research Tool
2024年7月16日 · Luciferase is a group of enzymes that oxidize a substrate known as luciferin to produce light. 1 For bioluminescence, the firefly luciferase enzyme catalyzes the oxidation of D-luciferin in the presence of oxygen (O 2), adenosine triphosphate (ATP), and …
Luciferase | Definition, Bioluminescence, Enzyme, Reaction,
5 天之前 · Luciferase, enzyme manufactured in the cells of certain organisms to control bioluminescence. The widespread bioluminescence of such living organisms as fireflies and bacteria is based on the oxidation of a luciferin molecule in …
Luciferase - an overview | ScienceDirect Topics
Luciferase is an enzyme found in fireflies that facilitates the conversion of luciferin to oxyluciferin in the presence of ATP, O2, and Mg2+, resulting in the emission of yellow light. It is commonly used to detect low levels of gene expression due to its …
Luciferase, an alternative to fluorescence - Integrated DNA …
2024年4月16日 · Luciferase enzymes regulate bioluminescence in cells and can be found in a variety of organisms. It provides a useful research tool that allows for the study of gene regulation, among other things, and offers an alternative method to fluorescent techniques. Check out this DECODED article to find out what it is and how it can be used in the lab.
Luciferase - an overview | ScienceDirect Topics
Luciferases are a heterogeneous group of oxidative enzymes that catalyze a reaction that produces an excited state intermediate that decays with the production of a photon.
Luciferase | Encyclopedia.com
2018年5月23日 · Luciferase — An enzyme that catalyzes the reaction between oxygen and luciferin. Luciferin — Complex carbon molecules that produce light when oxidized. Oxidation — The process where a molecule loses one or more electrons.
Luciferase - an overview | ScienceDirect Topics
Luciferase is a generic term for a family of photo-proteins that can be isolated from insects, marine organisms and prokaryotes [57]. Biochemically, all luciferases are oxygenases that use molecular oxygen to oxidize a substrate, with the formation of …
Illuminating insights into firefly luciferase and other …
Understanding luciferase enzymology and the structure of compounds that modulate luciferase activity can be used to improve the design of luminescence-based assays. This review provides an overview of these popular reporters with an emphasis on the ...
1001 lights: luciferins, luciferases, their mechanisms of ... - PubMed
2016年10月24日 · Bioluminescence (BL) is a spectacular phenomenon involving light emission by live organisms. It is caused by the oxidation of a small organic molecule, luciferin, with molecular oxygen, which is catalysed by the enzyme luciferase.