A video protocol for rapid dissection of mouse dorsal root ganglia …
2020年6月24日 · Dorsal root ganglia (DRG) are heterogeneous assemblies of assorted sensory neuron cell bodies found in bilateral pairs at every level of the spinal column. Pseudounipolar afferent neurons convert external stimuli from the environment into electrical signals that are …
- 作者: James Nicholas Sleigh, James Nicholas Sleigh, Steven J West, Giampietro Schiavo, Giampietro Schiavo
- 预计阅读时间：8 分钟
- Publish Year: 2020
A simple, step-by-step dissection protocol for the rapid isolation …
Located in the dorsal intervertebral foramen adjacent to the spinal cord, dorsal root ganglia (DRG) are heterogeneous collections of sensory neuron cell somas found in pairs at each level of the …
Protocol Protocol for dissection and culture of murine dorsal root ...
2021年3月19日 · Dorsal Root Ganglia (DRG) are located in the intervertebral foramen adjacent to the spinal cord. It is therefore necessary to dissect the vertebral column before isolating the …
- 作者: Caroline Perner, Caroline L. Sokol
- Publish Year: 2021
Protocol for dissection and culture of murine dorsal …
Timing: 5–10 min per mouse. Dorsal Root Ganglia (DRG) are located in the intervertebral foramen adjacent to the spinal cord. It is therefore necessary to dissect the vertebral column before isolating the DRG. To decrease blood …
Protocol for the isolation and culture of mouse dorsal root …
DRG fibers are lipid-rich, particularly the largest fibers associated with the lumbar DRG. If DRG fibers are not removed (Step 6e), this can add excess debris and glial cells to the cell …
A video protocol for rapid dissection of mouse dorsal root
2020年6月24日 · We have previously published a step-by-step dissection method for the rapid isolation of post-natal mouse DRG. Here, the objective is to extend the protocol by providing …
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(PDF) A simple, step-by-step dissection protocol for …
2016年12月1日 · The spinal cord and DRG tissues were taken after sodium pentobarbital anesthesia (50 mg/kg, intraperitoneal) in mice, which perfused intracardially with saline followed by 4% ice-cold ...
(PDF) A video protocol for rapid dissection of mouse …
2020年12月1日 · In addition, dorsal root ganglia (DRGs) and spinal cord were also isolated from TNBS animals (thoracic-lumbar portion, T10-L1 and lumbar-sacral portion, L6-S1) [74]. All samples collected were ...
A simple, step-by-step dissection protocol for the rapid ... - PubMed
2016年2月11日 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary …
Long-term imaging of dorsal root ganglia in awake behaving mice …
2019年7月12日 · In order to image DRG in the living mice, we custom designed a vertebral mount that could be firmly attached to the spinal column (Fig. 1a–c), while allowing access to the …
