Pull-Down Assays | Thermo Fisher Scientific - US
The pull-down assay is an in vitro method used to determine a physical interaction between two or more proteins. Pull-down assays are useful for both confirming the existence of a protein–protein interaction predicted by other research techniques (e.g., co-immunoprecipitation) and as an initial screening assay for identifying previously ...
Pull-down Assays - MilliporeSigma
Pull-down assays involve isolation of a protein complex by adsorbing the complex onto beads. Immobilized ligands on the beads bind specifically to a component of the complex, either via an affinity tag (e.g., GST, histidine, maltose binding protein, etc.) or an antibody.
Protein-Protein Interactions: Pull-Down Assays - PubMed
The pull-down assay is an in vitro technique used to detect physical interactions between two or more proteins and an invaluable tool for confirming a predicted protein-protein interaction or identifying novel interacting partners.
What is Pull-Down Assay Technology? - Creative Proteomics
Pull-down assay technology is a powerful biochemical technique used to study protein-protein interactions (PPIs) in vitro. It allows researchers to identify and characterize interactions between proteins and other biomolecules.
Pull Down Assays - NEB
Pull-down assays are commonly used to either confirm the presence of a scant protein species or enrich population for a particular protein, as well as to identify natural binding partners for the captured protein.
Protein–Protein Interactions: Pull-Down Assays | SpringerLink
2017年7月1日 · The pull-down assay is an in vitro technique used to detect physical interactions between two or more proteins and an invaluable tool for confirming a predicted protein–protein interaction or identifying novel interacting partners.
Co-Immunoprecipitation (Co-IP) and Pull-Down
The pull-down assay is a robust method by which a target protein is extracted from a mixture (e.g., cell lysate or serum) via its affinity to a specially prepared solid support.
RNA Pull Down: Principles, Probe Design, and Data Analysis
The basic principle of RNA Pull Down involves the isolation of RNA-binding proteins (RBPs) that interact with a specific RNA of interest. This technique utilizes biotinylated RNA probes that are complementary to the target RNA sequence.
Protein Pull-Down Techniques - MilliporeSigma
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
Single-molecule pull-down for studying protein interactions
2012年2月9日 · The assay combines the conventional pull-down assay with single-molecule total internal reflection fluorescence (TIRF) microscopy and allows the probing of single macromolecular complexes...
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