A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)
2022年7月21日 · The FLIM method Fluorescence lifetime imaging microscopy (FLIM) exploits the inherent properties of fluorescent dyes to produce an image. Fluorescent molecules have become essential tools for cell biologists. They are widely used in microscopy to visualize many different structures, targets and dynamic processes within cells, tissues, and even whole organisms.
Fluorescence - Leica Microsystems
Fluorescence lifetime imaging microscopy (FLIM) is an imaging technique that makes use of the inherent properties of fluorescent dyes. Aside from having characteristic emission spectra, each fluorescent molecule has a characteristic lifetime that reflects how long the fluorophore spends in the excited state before emitting a photon.
An Introduction to Fluorescence - Leica Microsystems
2023年6月2日 · This article gives an introduction to fluorescence and photoluminescence, which includes phosphorescence, explains the basic theory behind them, and how fluorescence is used for microscopy.
Fluorescence in Microscopy | Science Lab | Leica Microsystems
2011年4月27日 · Fluorescence microscopy is a special form of light microscopy. It uses the ability of fluorochromes to emit light after being excited with light of a certain wavelength. Proteins of interest can be marked with such fluorochromes via antibody staining or …
Fluorescent Dyes | Science Lab | Leica Microsystems
2023年11月15日 · A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP – genetically linked to the protein of interest. If cloning is impossible – for instance in histologic samples – techniques such as immunofluorescence staining are used to …
Introduction to Widefield Microscopy - Leica Microsystems
2017年6月29日 · This article gives an introduction to widefield microscopy, one of the most basic and commonly used microscopy techniques. It also shows the basic differences between widefield and confocal microscopy.
How to Prepare your Specimen for Immunofluorescence Microscopy
2022年1月10日 · How to Prepare your Specimen for Immunofluorescence Microscopy Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures.
Epi-Illumination Fluorescence and Reflection-Contrast Microscopy ...
2023年11月2日 · Fluorescence microscopy had been used for many years with only transmitted light and darkfield illumination. Over time, the demand grew for improved illumination and it led to the development of epi-illumination, also called incident illumination. After 4 decades of development and improvement, epi-illumination fluorescence microscopy became a practical method for routine lab work and research ...
What is FLIM - Fluorescence Lifetime Imaging Microscopy?
2022年10月17日 · FLIM exploits fluorescence lifetime which is a measure of how long a fluorescent molecule or fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon. The emission of a fluorescence photon from a fluorophore does not always occur at an exact time after excitation. Instead, a distribution of times is observed which can be ...
Milestones in Incident Light Fluorescence Microscopy - Leica …
2017年3月6日 · Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the help of fluorescence molecules is a standard method in nearly every life science discipline today. This broad application range goes back to the technical work of some researchers who wanted to ...